Daylesford Scouts closed last year because they could not find leaders.

Now, the only remaining Girl Guide leader in Creswick, Ms Doyle, warned the group could fold.

She said the Guides had gone into recess two weeks ago and would have to close permanently if no help was found.

Legally, they need two leaders to run a unit.

In the past three months, Ms Doyle had lost two leaders after one bought a business and the other moved to Melbourne.

She has not been able to find adults in the community to replace them.

The Creswick branch of the Girl Guides has been running for 60 years.

Ms Doyle said there had been just one other recess in the history of the group, when in the 1960s one of the leaders' husbands had died.

The program was only closed for six months.

Ms Doyle has been involved with Girl Guides for 42 years and became a leader in Creswick two years ago.

She said activities ranged from learning how to survive in the bush to planting trees.

"There are no other youth activities in Creswick except for sport," Ms Doyle said.

"Daylesford Scouts closed last year because they couldn't get any leaders and now they've got bigger problems with more kids roaming the streets and damaging things then ever before."

She said Girl Guides was unique because it focused on providing care to females and the leaders were often role models to the girls who participated.

There were currently 12 girls in the Creswick group between the ages of five and 14.